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KMID : 0384119930130040689
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 4 p.689 ~ p.698
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Cytogenetic Studies in Chronic Myelogenous Leukemia
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Abstract
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To investigate chromosomal abnormalities of chronic myelogenous leukemia (CML), we studied G-banded chromosome using Wright stain trypsin treatment from peripheral blood and/or bone marrow in 31 patients during one year period from August 1991 to
July
1992. The culture methods used were methotrexate synchronization, short term culture with ethidium bromide condensation inhibition and unsynchronized 12-16 hour colcemid treatment. Among 31 CML patients, Philadelphia chromosome-positive CML
[Ph(+)
CML]
were observed in 29 patients (93.5%), of which 28 patients were classic from of t(9;22)(q34;q11) and one patient was a variant form of t(17;22)(p13;q11), and Ph(-) CML were observed in two patients (6.5%). Among Ph(+) CML patients, four (19.0%)
of
21
patients studied during chronic phase, both of two patients studied accelerated phase and five(71.4%) of seven patients studied during blastic crisis had additional secondary chromosomal abnormalities. And all the three patients who were studied
twice
with 3-8 month interval, had secondary chromosomal abnormalities not at the first studies but the second studies. Secondary chromosomal abnormalities found in Ph(+) CML during chronic phase were rather simple ones such as extra Ph, loss or gain
of
one
or two chromosome(s), but those in blastic crisis were very complex ones such as t(9;12;22), or three clones with complex translocation. Relatively common secondary chromosomal abnormalities were abnormalities of chromosome 1, extra-Ph and the
loss
of Y
chromosome.
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KEYWORD
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